Part:BBa_K2924019:Design

Promoter AR with the reporter gene sfGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 291
  

Design Notes

The promoter is sensitive to long chain fatty acids and can be used to quantify them by measuring the GFP fluorescence. This part is an improvement of BBa_K2581012. This part is more sensitive, with higher dose responses to fatty acids, than the original part.

Source

The DNA sequenced were obtained from the registry basic parts and synthesized by IDT.